Journal: Biological Research

Article Title: Female prediabetic rats are protected from vascular dysfunction: the role of nitroso and sulfide signaling

doi: 10.1186/s40659-024-00575-1

Figure Lengend Snippet: Gene and protein expressions of inflammatory factors in female Wistar and HTG rats

Article Snippet: Afterward, the membranes were incubated with a rabbit polyclonal anti-eNOS antibody (Abcam, Cambridge, UK; dilution 1:1000), a rabbit polyclonal anti-iNOS antibody (Proteintech ® , Manchester, UK; dilution 1:1000), a rabbit polyclonal anti-CBS antibody (Proteintech ® , Manchester, UK; dilution 1:3000), a mouse monoclonal anti-CSE antibody (Proteintech ® , Manchester, UK; dilution 1:5000), a mouse monoclonal anti-TNFα antibody (Proteintech ® , Manchester, UK; dilution 1:3000), a mouse monoclonal anti-NFκB antibody (Cell Signaling, Danvers, MA, USA; dilution 1:1000), a rabbit polyclonal anti-Mcp-1 antibody (Abcam, Cambridge, UK; dilution 1:1000), a rabbit polyclonal anti-ICAM antibody (Proteintech ® , Manchester, UK; dilution 1:1000), or a rabbit polyclonal anti-COX2 antibody (Proteintech ® , Manchester, UK; dilution 1:500) overnight at 4 °C.

Techniques:

Journal: Cell reports

Article Title: Lactic acid induces transcriptional repression of macrophage inflammatory response via histone acetylation

doi: 10.1016/j.celrep.2024.113746

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal biotinylated anti-mouse TNF-α , eBioscience , Cat# 13-7341-81; RRID: AB_466950.

Techniques: Recombinant, Luciferase, Reporter Assay, Staining, Control, Software

Journal: Microorganisms

Article Title: Adenoviral Vector Codifying for TNF as a Co-Adjuvant Therapy against Multi-Drug-Resistant Tuberculosis

doi: 10.3390/microorganisms11122934

Figure Lengend Snippet: Gene expression of pro-inflammatory cytokines in the different experimental groups. Gene expression quantification by qPCR using the 2^ (−ΔCt) method relative to Rplp0 (housekeeping gene) of ( A ) Ifng , ( B ) Tnf and ( C ) Nos2 at day 14, 28 and 60 of treatment with AdTNF, AdGFP or SS. At day 60 of treatment, a significant increase in gene expression was induced by AdTNF treatment. Asterisks represent statistical significance (**** p < 0.0001, two-way ANOVA).

Article Snippet: Cytokine detection was performed with goat anti-mouse polyclonal antibodies against TNF, IFN-γ and iNOS (Santa Cruz Biotechnology, Santa Cruz, CA, USA), secondary anti-goat antibody-HRP (Goat-on-Rodent HRP-Polymer, Biocare Medical); finally, slides were revealed with diaminobenzidine/H 2 O 2 and contrasted with hematoxylin stain.

Techniques: Gene Expression

Journal: Microorganisms

Article Title: Adenoviral Vector Codifying for TNF as a Co-Adjuvant Therapy against Multi-Drug-Resistant Tuberculosis

doi: 10.3390/microorganisms11122934

Figure Lengend Snippet: Percentage of immunostained cells to pro-inflammatory cytokines and iNOS in pneumonic areas of the different experimental groups. Percentage of immunostained cells to ( A ) IFN-γ, ( B ) TNF and ( C ) iNOS in the pneumonic areas of mice infected with MDR-TB after the indicated days of treatment with one dose of AdTNF, AdGFP or SS administered by intratracheal route. AdTNF induced a significant increment of immunostained cells in all the evaluated time points. Asterisks represent statistical significance (**** p < 0.0001, two-way ANOVA). ( D ) Representative microphags (40×) of immunostaining detection (brown cytoplasm peroxidase staining) of the indicated cytokine and iNOS in pneunmonic areas comparing AdTNF and AdGFP.

Article Snippet: Cytokine detection was performed with goat anti-mouse polyclonal antibodies against TNF, IFN-γ and iNOS (Santa Cruz Biotechnology, Santa Cruz, CA, USA), secondary anti-goat antibody-HRP (Goat-on-Rodent HRP-Polymer, Biocare Medical); finally, slides were revealed with diaminobenzidine/H 2 O 2 and contrasted with hematoxylin stain.

Techniques: Infection, Immunostaining, Staining